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Journal
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Neuroscience
/
分子分析的谷类区域解剖
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Cerebellar Regional Dissection for Molecular Analysis
Please note that all translations are automatically generated.
Click here for the English version.
分子分析的谷类区域解剖
DOI:
10.3791/61922-v
•
08:51 min
•
December 05, 2020
•
Katherine A. Hamel
,
Marija Cvetanovic
1
Department of Neuroscience
,
University of Minnesota
Chapters
00:04
Introduction
00:54
Brain Extraction and Dissection
04:42
RNA Extraction
07:01
Results: RT qPCR Relative Gene Expression in Isolated Specific Regions of the Cerebellum
08:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
不同的小脑区域被牵连到不同的行为输出中发挥作用,但潜在的分子机制仍然未知。本文描述了一种可重复和快速解剖半球、前部和后部区域以及深小脑核的方法,以便通过分离RNA和测试基因表达差异来探测分子差异。
Tags
Cerebellum
Cerebellar Regions
Molecular Analysis
Dissection
Brain Regions
Brain Matrix
Vermis
Cerebellar Cortex
Deep Cerebellar Nuclei
Euthanized Mouse
Brain Extraction
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