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JoVE Journal
Chemistry
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通过质谱学研究蛋白质高阶结构,与二乙基碳酸酯共价标签
 
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通过质谱学研究蛋白质高阶结构,与二乙基碳酸酯共价标签

Article DOI: 10.3791/61983-v 10:36 min June 15th, 2021
June 15th, 2021

Summary

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Click here for the English version.

介绍了在质谱检测中进行基于二乙基碳酸酯的共价贴标的实验程序。二甲基碳酸酯只是与感兴趣的蛋白质或蛋白质复合物混合,导致溶剂可获取氨基酸残留物的修饰。经蛋白解消化和液相色谱/质谱分析后,可识别修改后的残留物。

Tags

化学,第172期,质谱,共价标签,蛋白质高阶结构,蛋白质-脂质复合物,蛋白质复合物,二乙基碳酸酯,溶剂可访问表面积
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