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Journal
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Biochemistry
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网站特定赖氨酸乙酰化的组蛋白为核体重组使用遗传密码扩展在
埃斯切里西亚大肠杆菌
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in
Escherichia coli
Please note that all translations are automatically generated.
Click here for the English version.
网站特定赖氨酸乙酰化的组蛋白为核体重组使用遗传密码扩展在
埃斯切里西亚大肠杆菌
DOI:
10.3791/62113-v
•
07:26 min
•
December 26, 2020
•
Chesley Marie Rowlett
,
Wenshe Ray Liu
1
Department of Chemistry
,
Texas A&M University
Chapters
00:04
Introduction
00:34
Acetylated Histone Protein Expression
03:13
Assembly of Histone Octamer
04:34
Nucleosome Assembly
06:04
Results: Histone Dimers, Tetramers, Octamers, and Assembled his Tagged and TEV Digested Nucleosomes
06:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出一种方法来表达使用遗传密码膨胀和组装重组核体体外的自负型高石蛋白。
Tags
Lysine Acetylation
Histones
Nucleosome Reconstitution
Genetic Code Expansion
Escherichia Coli
Mononucleosome
Epigenetic Experiments
Biochemical Assays
Binding Assays
Structure Determination
Histone Lysis Buffer
Inclusion Bodies
Nickel-NTA Purification
Acetylated Histone Protein
Dialysis
Acetic Acid Buffer
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