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三维类器官和球状体模型的染色和高分辨率成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
Please note that all translations are automatically generated.
Click here for the English version.
三维类器官和球状体模型的染色和高分辨率成像
DOI:
10.3791/62280-v
•
07:35 min
•
March 27, 2021
•
Alejandro Lopez Gonzalez*
1
,
Léa Luciana*
1
,
Clémentine Le Nevé
,
Julie Valantin
,
Laura Francols
,
Nicolas Gadot
,
Christophe Vanbelle
,
Laurianne Davignon
,
Laura Broutier
1
Childhood Cancer & Cell Death (C3)
,
Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon 69373
,
2
Plateforme Anatomopathologie Recherche
,
Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL)
,
3
Plateforme d’Imagerie cellulaire, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL)
,
4
PerkinElmer S.A.S.
Chapters
00:04
Introduction
01:05
3D Whole Mount Staining
03:09
Embedding of Large 3D Cell Culture Models
05:19
Embedding of Small 3D Cell Culture Models
06:26
Results: High‐Resolution Imaging of 3D Structures and Spheroid Models
07:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提供了详细、稳健和互补的方案,对从 100 μm 到几毫米的固定三维细胞培养模型进行染色和亚细胞分辨率成像,从而能够可视化它们的形态、细胞类型组成和相互作用。
Tags
3D In Vitro Models
Organoids
Spheroids
Whole Mount Staining
Subcellular Resolution Imaging
Permeabilization
Primary Antibody
Secondary Antibody
Hoechst 33342
3D Structure Manipulation
Pipetting
Centrifugation
Gentle Agitation
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