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Microscopia a scansione laser confocale della dinamica del calcio in fette di tessuto pancreatico di topo acuto
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Microscopia a scansione laser confocale della dinamica del calcio in fette di tessuto pancreatico di topo acuto

Article DOI: 10.3791/62293-v • 10:49 min • April 13th, 2021
April 13th, 2021 •
1, 1,2, 1, 1, 1,3, 1
1Institute of Physiology, Faculty of Medicine, University of Maribor, 2Faculty of Natural Sciences and Mathematics, University of Maribor, 3Center for Physiology and Pharmacology, Medical University of Vienna

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Presentiamo la preparazione di fette di tessuto pancreatico acuto e il loro utilizzo nella microscopia a scansione laser confocale per studiare la dinamica del calcio contemporaneamente in un gran numero di cellule vive, per lunghi periodi di tempo e con alta risoluzione spaziotemporale.

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Biologia Numero 170 Pancreas agarosio fetta di tessuto preparazione in situ microscopia a scansione laser confocale imaging del calcio risoluzione unicellulare 3R
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