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Journal / Biochemistry / Forberedelse af prøvestøttefilm i…
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Biochemistry

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JoVE Journal Biochemistry
Forberedelse af prøvestøttefilm i transmissionselektronmikroskopi ved hjælp af en støtteflådsblok
Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
Journal (Biochemistry)
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Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Journal (Biochemistry)
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
 
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Forberedelse af prøvestøttefilm i transmissionselektronmikroskopi ved hjælp af en støtteflådsblok

Article DOI: 10.3791/62321-v • 06:10 min • April 8th, 2021
April 8th, 2021 •
1, 1, 1
1Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, South Kensington Campus

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Prøveforberedelse til kryo-elektronmikroskopi (cryo-EM) er en betydelig flaskehals i strukturbestemmelsesarbejdsgangen for denne metode. Her giver vi detaljerede metoder til brug af en brugervenlig, tredimensionelt trykt blok til fremstilling af støttefilm for at stabilisere prøver til transmission EM-undersøgelser.

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Biokemi TEM gitter forberedelse støtte film amorf kulstof grafen grafenoxid flydeblok
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