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Journal
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Immunology and Infection
/
聚焦激光扫描显微镜为基础的定量分析
阿斯珀吉卢斯熏蒸
孔尼迪亚分布在全山光学清除鼠肺
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of
Aspergillus fumigatus
Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
Please note that all translations are automatically generated.
Click here for the English version.
聚焦激光扫描显微镜为基础的定量分析
阿斯珀吉卢斯熏蒸
孔尼迪亚分布在全山光学清除鼠肺
DOI:
10.3791/62436-v
•
15:01 min
•
September 18, 2021
•
Ivan V. Maslov
,
Andrey O. Bogorodskiy
,
Mariia V. Pavelchenko
,
Ilia O. Zykov
,
Natalya I. Troyanova
,
Valentin I. Borshchevskiy
3
,
Marina A. Shevchenko
1
Research Center for Molecular Mechanisms of Aging and Age-Related Diseases
,
Moscow Institute of Physics and Technology
,
2
Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
,
Russian Academy of Sciences
,
3
Institute of Biological Information Processing (IBI-7: Structural Biochemistry)
,
Forschungszentrum Jülich
Chapters
00:04
Introduction
01:03
Aspergillus fumigatus
Conidia Application & Specimen Preparation
01:22
Mouse Lung Lobe Optical Clearing
02:23
Mouse Lung Lobe Imaging with CLSM
06:50
Image Processing: Surface Rendering
09:23
Image Processing: Mask Correction
12:54
Conidia Quantitative Analysis
13:41
Representative Results
14:07
Conclusions
Summary
Automatic Translation
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Automatic Translation
我们描述了对小鼠气道中
阿斯珀吉卢斯·福米加图斯
孔尼迪亚(3微米大小)分布进行定量分析的方法。该方法还可用于分析各种病理条件模型中气道中的微粒和纳米粒子聚集分布。
Tags
Confocal Laser Scanning Microscopy
Aspergillus Fumigatus
Conidia
Optical Clearing
Mouse Lung
Quantitative Analysis
Respiratory Tract
Mucociliary Clearance
Alveolar Macrophages
Neutrophils
Optical Clearing
Benzyl Alcohol
Benzyl Benzoate
Confocal Microscopy
Laser
Spectral Range
Detector Gain
Pinhole
Pixel Resolution
Z-stack
Article
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