JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Biology
/
激光微辐射研究S阶段DNA招募
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Laser Micro-Irradiation to Study DNA Recruitment During S Phase
Please note that all translations are automatically generated.
Click here for the English version.
激光微辐射研究S阶段DNA招募
DOI:
10.3791/62466-v
•
07:11 min
•
April 16, 2021
•
Bearach Miwatani-Minter
2
,
Gergely Rona
2,3
1
Department of Biochemistry and Molecular Pharmacology
,
New York University School of Medicine
,
2
Laura and Isaac Perlmutter Cancer Center
,
New York University School of Medicine
,
3
Howard Hughes Medical Institute
,
New York University School of Medicine
Chapters
00:04
Introduction
00:47
Preparation of Cells for Micro-Irradiation
01:18
Selecting S Phase Cells for Imaging
02:40
Micro-Irradiation for Immunofluorescence Staining or Time Lapse Imaging
03:44
Time Lapse Imaging
04:47
Results: DNA Recruitment During S Phase After Micro-Irradiation
06:23
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了一种非侵入性方法,用于有效识别下游显微镜研究的S相细胞,例如通过激光微辐射测量DNA修复蛋白的招募。
Tags
Laser Micro-irradiation
DNA Repair
S Phase
PCNA
Spatiotemporal Resolution
DNA Damage Repair Protein Kinetics
Confocal Imaging
Olaparib
Environmental Chamber
Microscope
MPlum
Binary Lines
ROI
ND Stimulation
Time Schedule
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
测试的速度和运动方向的视觉灵敏度在蜥蜴
一种高通量的方法,在全球范围内研究在酿酒酵母中的细胞器形态
成像错配修复和DNA损伤的细胞反应
枯草芽孢杆菌</em
激光烧蚀斑马鱼Pronephros研究肾小管上皮再生
确定的能源系统,在运动的贡献
液相亲和捕获试验用磁珠研究蛋白质相互作用:脊髓灰质炎病毒的Nanobody范例
结合单分子操纵和成像蛋白-DNA相互作用的研究
样机小型化流式细胞仪及配套的混合微流控技术降低重力环境硬件演示
使用酿
酒酵母
中的5-乙炔基-2'-脱氧尿苷掺入测定S相持续时间
使用延时显微镜和蛋白质的阶段特异性核耗竭来研究
酿酒酵母
的减数分裂
Read Article