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Journal
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Biology
/
リンゴパルプでFluo-4/AMで細胞質
Ca2+
を染色する
JoVE Journal
Biology
Author Produced
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JoVE Journal
Biology
Staining the Cytoplasmic Ca
2+
with Fluo-4/AM in Apple Pulp
Please note that all translations are automatically generated.
Click here for the English version.
リンゴパルプでFluo-4/AMで細胞質
Ca2+
を染色する
DOI:
10.3791/62526-v
•
08:05 min
•
November 06, 2021
•
Lina Qiu
,
Daqing Huang
,
Yongzhang Wang
,
Haiyong Qu
1
College of Horticulture
,
Qingdao Agricultural University
Chapters
00:03
Introduction
00:57
Protoplast Extraction
01:49
Small-molecule Calcium Ion Fluorescence Staining
04:46
Protoplast Viability Assay
05:48
Results
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
リンゴパルプ細胞の単離プロトプラストは、細胞質
Ca2+
濃度を検出するためにカルシウム蛍光試薬を装填した。
Tags
Cytoplasmic Ca2+
Fluo-4/AM
Apple Pulp
Calcium Imaging
Protoplast Extraction
Small Molecule Fluorescent Indicator
Calcium Ion Selectivity
Esterification Incubation
Enzymatic Solution
Fluorescence Microscope
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