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BrdU-DNA標識と細胞周期判別イメージングを用いたグローバルDNA二本鎖末端切除の評価
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JoVE Journal
Biology
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Please note that all translations are automatically generated.
Click here for the English version.
BrdU-DNA標識と細胞周期判別イメージングを用いたグローバルDNA二本鎖末端切除の評価
DOI:
10.3791/62553-v
•
06:44 min
•
April 28, 2021
•
Julia O’Sullivan*
1,2
,
Sofiane Y. Mersaoui*
1,2
,
Guy Poirier
3
,
Jean-Yves Masson
2
1
Oncology Division
,
Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion
,
2
Department of Molecular Biology, Medical Biochemistry, and Pathology
,
Laval University Cancer Research Center
,
3
Oncology Division
,
CHU de Québec Research Center, CHUL Pavilion
Chapters
00:05
Introduction
00:37
Cell Culture, Treatments, and Coverslip Preparation
01:18
Pre-Extraction and Fixation
02:13
Permeabilization and Immunostaining
03:53
Image Analysis
04:16
Results: Visualization of DNA Double-Strand End Resection During S to G2 Phase of the Cell Cycle Using an Immunofluorescence-Based Method
06:05
Conclusion
Summary
Automatic Translation
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Automatic Translation
本プロトコールでは、細胞周期のS/G2期におけるDNA二本鎖末端切除を免疫蛍光ベースの方法を用いて可視化する方法を実証する。
Tags
DNA Double-strand Break
DNA Resection
Cell Cycle
BrdU Labeling
Immunostaining
Imaging
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