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JoVE Journal Neuroscience
Fluorescensaktiveret cellesortering-radioligandbehandlet væv (FACS-RTT) til bestemmelse af den cellulære oprindelse af radioaktivt signal
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Fluorescensaktiveret cellesortering-radioligandbehandlet væv (FACS-RTT) til bestemmelse af den cellulære oprindelse af radioaktivt signal

Article DOI: 10.3791/62883-v • 12:04 min • September 10th, 2021
September 10th, 2021 •
1,2, 1,3,4, 1,5, 1,2, 1,2
1Division of Adult Psychiatry, Department of Psychiatry, University Hospitals of Geneva, 2Department of Psychiatry, University of Geneva, 3Division of Nuclear medicine and Molecular Imaging, University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology, University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine, Imperial College London

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Fluorescensaktiveret cellesortering-radioligandbehandlet væv (FACS-RTT) er et kraftfuldt værktøj til at studere rollen som 18 kDa translocatorprotein eller Serotonin 5HT 2A-receptorekspression i Alzheimers sygdom i cellulær skala. Denne protokol beskriver ex vivo-anvendelsen af FACS-RTT i TgF344-AD-rottemodellen.

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Neurovidenskab udgave 175 Alzheimers sygdom Fluorescensaktiveret cellesortering til radioligand slidt væv TgF344-AD Positronemissionstomografi / Enkelt fotonemission Computertomografi TSPO 5HT2AR
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