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在微流体装置中生成具有功能性神经肌肉接头的人类运动单元
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
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在微流体装置中生成具有功能性神经肌肉接头的人类运动单元

DOI:
10.3791/62959-v

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10:48 min

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September 07, 2021

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, , , , , , , , , , , ,
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute,KU Leuven – University of Leuven, 2VIB Bio Imaging Core,KU Leuven – University of Leuven, 3Department of Development and Regeneration, Stem Cell and Developmental Biology,KU Leuven – University of Leuven, 4Department of Neurology,University Hospitals Leuven

Chapters

  • 00:05Introduction
  • 00:54Pre-Assembled Microfluidic Device Preparation
  • 02:39Silicone Microfluidic Device Preparation
  • 05:18Neural Progenitor Cell Plating in Microfluidic Devices
  • 07:15Human Primary Mesoangioblast (MAB) Plating in Microfluidic Devices
  • 08:32Results: Generation of NMJs in Microfluidic Devices
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种在市售微流体装置中产生人类运动单元的方法，方法是将人诱导多能干细胞衍生的运动神经元与人类原代介观细胞衍生的肌管共同培养，从而形成功能活性的神经肌肉接头。

Tags

Motor UnitsNeuromuscular JunctionsMicrofluidic DevicesStem Cell TechnologyMotor Neuron DisorderAmyotrophic Lateral SclerosisPoly-L-ornithineLamininNeurobasal MediumSCM Sheets

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