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Three-Dimensional Motor Nerve Organoid Generation
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Three-Dimensional Motor Nerve Organoid Generation

Three-Dimensional Motor Nerve Organoid Generation

DOI:
10.3791/61544-v

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09:57 min

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September 24, 2020

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, , , , , ,
1Institute of Industrial Science,The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, 3Faculty of Science and Engineering,Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc.

Chapters

  • 00:05Introduction
  • 00:47Photolithographic SU-8 Mold Fabrication
  • 01:59Polydimethlysiloxane (PDMS) Microfluidic-Based Tissue Culture Chip Fabrication
  • 02:43PDMS Chip Preparation for Motor Nerve Organoid (MNO) Formation
  • 03:40Induced Pluripotent Stem (iPS) Cell Differentiation
  • 06:39Motor Nerve Organoid Formation
  • 07:57Results
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides a comprehensive procedure to fabricate human iPS cell-derived motor nerve organoid through spontaneous assembly of a robust bundle of axons extended from a spheroid in a tissue culture chip.

Tags

Keywords: 3D Motor Nerve OrganoidIPS CellsAxon BundlesMotor Neuron DiseasesSU-8 PhotoresistPDMS ChipBasement Membrane MatrixDMEM/F12

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