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Neuroscience

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JoVE Journal Neuroscience
Geração de Unidades Motoras Humanas com Junções Neuromusculares Funcionais em Dispositivos Microfluidos
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Geração de Unidades Motoras Humanas com Junções Neuromusculares Funcionais em Dispositivos Microfluidos

Article DOI: 10.3791/62959-v • 10:48 min • September 7th, 2021
September 7th, 2021 •
1,2, 1,2, 1,2, 1,2, 3,4, 3,4, 5, 3,4, 1,2, 1,2, 5, 1,2,6, 1,2
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Center for Brain & Disease Research, Laboratory of Neurobiology, , 3VIB Center for Brain & Disease Research, Research Group Molecular Neurobiology, , 4VIB Bio Imaging Core, KU Leuven - University of Leuven, 5Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 6Department of Neurology, University Hospitals Leuven

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Descrevemos um método para gerar unidades motoras humanas em dispositivos microfluidos comercialmente disponíveis, co-culminando neurônios motores pluripotentes induzidos por células-tronco com miótubos primários derivados do mesoangioblast, resultando na formação de junções neuromusculares funcionalmente ativas.

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Neurociência Edição 175
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