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Bioengineering
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基于人类原发性皮肤成纤维细胞的亨廷顿舞蹈症模型微管加端动力学可视化
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Bioengineering
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JoVE Journal
Bioengineering
Microtubule Plus-End Dynamics Visualization in Huntington’s Disease Model based on Human Primary Skin Fibroblasts
Please note that all translations are automatically generated.
Click here for the English version.
基于人类原发性皮肤成纤维细胞的亨廷顿舞蹈症模型微管加端动力学可视化
DOI:
10.3791/62963-v
•
10:38 min
•
January 08, 2022
•
Aleksandra Taran*
1,2
,
Lilia Belikova (Shuvalova)*
2,3
,
Svetlana Lavrushkina
2
,
Alexandra Bogomazova
4
,
Maria Lagarkova
4
,
Irina Alieva
3
1
A.N. Belozersky Institute of Physico-Chemical Biology
,
Lomonosov Moscow State University
,
2
Faculty of Bioengineering and Bioinformatics
,
Lomonosov Moscow State University
,
3
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
,
4
Center for Precision Genome Editing and Genetic Technologies for Biomedicine
,
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Chapters
00:05
Introduction
00:44
Protocol
01:02
Obtaining a Primary Culture of Human Skin Fibroblasts
02:14
Cell Cultivation
04:45
Transfection
06:46
Preparing for Imaging
08:08
Setting the Imaging Parameters
09:45
Results
10:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议专门用于通过EB3蛋白转染进行微管加端可视化,以研究它们在原代细胞培养物中的动态特性。该协议是在从亨廷顿舞蹈症患者获得的人类原发性皮肤成纤维细胞上实施的。
Tags
Microtubule Dynamics
EB3 Protein
Huntington’s Disease
Human Primary Skin Fibroblasts
Cell Culture
Transfection
Fluorescent Labeling
Microscopy
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