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Isolering af epitelceller fra human tandsække
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JoVE Journal Biology
Isolation of Epithelial Cells from Human Dental Follicle

Isolering af epitelceller fra human tandsække

DOI:
10.3791/63104-v

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04:07 min

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November 05, 2021

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1Department of Conservative Dentistry,Kyung Hee University Dental Hospital at Gangdong, 2Department of Conservative Dentistry, School of Dentistry,Kyung Hee University

Chapters

  • 00:04Introduction
  • 00:34Isolate Single-Cell Populations from Dental Follicle
  • 02:52Results: Emergence of Epithelial Cells
  • 03:13Conclusion

Summary

Automatic Translation

Automatic Translation

Tandsækken indeholder en epitelpopulation og mesenkymale celler. Epitelpopulationen blev udvalgt blandt den heterogene dentalsækkecellepopulation ved at give et særskilt kulturmedium. Epitelceller overlevede og dannede kolonier i et serumfrit medium.

Tags

Dental FollicleEpithelial CellsCell IsolationCell CultureCollagenaseProteaseTrypsin-EDTAKeratinocyte MediumCell StrainerCentrifugationCell MorphologyCell Colonies

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