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蓝藻中完整藻胆体的分离与表征
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
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蓝藻中完整藻胆体的分离与表征
DOI:
10.3791/63272-v
•
06:26 min
•
November 10, 2021
•
Han-Wei Jiang
,
Ming-Yang Ho
2
1
Department of Life Science
,
National Taiwan University
,
2
Institute of Plant Biology
,
National Taiwan University
Chapters
00:04
Introduction
00:38
Cell Culture and Harvesting
01:13
Cells Lysis
02:43
Preparation of the Sucrose Gradient Buffers and PBS Isolation
03:32
Measurement of PBS Fluorescence at 77K
04:00
Results: Spectral Evaluation of Phycobilisomes
05:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
目前的方案详细介绍了通过不连续蔗糖密度梯度离心从蓝藻中分离藻胆体的过程。完整的藻糖体的分数通过77K荧光发射光谱和SDS-PAGE分析得到证实。所得的藻胆体组分适用于TEM的负染色和质谱分析。
Tags
Phycobilisome
Cyanobacteria
Isolation
Characterization
Protocol
B-HEPES Medium
Potassium Phosphate Buffer
Cell Lysis
Triton X-100
Sucrose Gradient Centrifugation
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