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Isolatie en karakterisering van intact fycobilisoom in cyanobacteriën
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Isolatie en karakterisering van intact fycobilisoom in cyanobacteriën

Article DOI: 10.3791/63272-v • 06:26 min • November 10th, 2021
November 10th, 2021 •
1, 1,2
1Department of Life Science, National Taiwan University, 2Institute of Plant Biology, National Taiwan University

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Het huidige protocol beschrijft de isolatie van fycobilisomen uit cyanobacteriën door centrifugering door middel van een discontinue sucrosedichtheidsgradiënt. De fracties van intacte fycobilisomen worden bevestigd door 77K fluorescerend emissiespectrum en SDS-PAGE-analyse. De resulterende fycobilisoomfracties zijn geschikt voor negatieve kleuring van TEM en massaspectrometrie-analyse.

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Biology Cyanobacterium phycobilisome Synechocystis sp. PCC 6803 Leptolyngbya sp. JSC-1 kraalklopper homogenisator discontinue sucrose dichtheid gradiënt 77K fluorescentie emissie spectrum
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