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使用带有可更换盖子的乳腺成像窗口进行纵向活体腔内显微镜检查
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
Please note that all translations are automatically generated.
Click here for the English version.
使用带有可更换盖子的乳腺成像窗口进行纵向活体腔内显微镜检查
DOI:
10.3791/63326-v
•
10:53 min
•
January 20, 2022
•
Larissa Mourao
2
,
Marta Ciwinska
2
,
Jacco van Rheenen
,
Colinda L. G. J. Scheele
2
1
Center for Cancer Biology
,
VIB (BE)
,
2
Department of Oncology
,
KU Leuven (BE)
,
3
Netherlands Cancer Institute, Department of Molecular Pathology
,
Oncode Institute (NL)
Chapters
00:05
Introduction
01:03
Preparation of the R.MIW
02:13
R.MIW Implantation
05:19
Repeated Intravital Microscopy Through the R.MIW
07:03
Opening and Closing the Lid of the R.MIW in Between Imaging Sessions
07:52
Results: Multi-Day IVM of Mammary Gland Remodeling
10:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种带有可更换盖子(R.MIW)的新型乳腺成像窗口。植入R.MIW后的活体显微镜检查允许在不同发育阶段以细胞分辨率对健康和患病的乳腺进行纵向和多日成像。
Tags
Mammary Imaging Window
Longitudinal Intravital Microscopy
Mammary Gland
Mammary Tumor
Single-cell Imaging
Cyanoacrylate Adhesive
Glass Cover Slip
Surgical Procedure
Region-of-interest
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