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Developmental Biology
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胚盤胞の開発と移植をモデル化するためのヒト芽球イドのプロトコル
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
Please note that all translations are automatically generated.
Click here for the English version.
胚盤胞の開発と移植をモデル化するためのヒト芽球イドのプロトコル
DOI:
10.3791/63388-v
•
12:09 min
•
August 10, 2022
•
Harunobu Kagawa*
1
,
Alok Javali*
1
,
Heidar Heidari Khoei*
1
,
Theresa Maria Sommer
3
,
Giovanni Sestini
3
,
Maria Novatchkova
,
Yvonne Scholte op Reimer
,
Nicolas Rivron
1
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
,
Vienna Biocenter (VBC)
,
2
Institute of Molecular Pathology (IMP)
,
Vienna Biocenter (VBC)
,
3
Vienna BioCenter PhD Program
,
Doctoral School of the University of Vienna and Medical University of Vienna
Chapters
00:04
Introduction
01:24
Formation of Naive PSC Aggregates
04:57
Blastoid Development
05:49
Formation of Blastoids in 96-well Ultra-low Attachment Microplates
07:05
Formation of Trophospheres
08:05
Results: Blastoid and Trophospheres Derived from Aggregates of Naïve hPSC
11:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
胚盤胞様細胞を効率的、タイムリーに、かつ逐次的に生成するヒト芽球の形成を概説するプロトコル。
Tags
Human Blastoids
Blastocyst Development
Implantation
In Vitro Fertilization
Pre-clinical Modeling
Therapeutic Targets
Non-hormonal Contraceptives
MEF Exclusion
PXGL Media
N2B27 Basal Media
Aggregation Media
Cell Detachment
MicroWells
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