/
/
In vivo Avbildning av biologisk vev med kombinert to-foton fluorescens og stimulert ramanspredningsmikroskopi
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Electronic and Computer Engineering,The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience,The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health,The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center,The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences,Southern University of Science and Technology
Chapters
- 00:04Introduction
- 00:59Starting-Up the Lasers
- 02:02Optical Alignment of the Combined TPEF-Stimulated Raman Scattering Microscope
- 04:39Optimizing Imaging Conditions
- 06:36In Vivo TPEF-SRS Imaging of Mouse Spinal Cord
- 08:03Results: In Vivo Dual-Modal Imaging of Spinal Axons and Myelin Sheaths
- 08:31Conclusion
Stimulert Raman-spredning (SRS) mikroskopi tillater etikettfri avbildning av biomolekyler basert på deres iboende vibrasjon av spesifikke kjemiske bindinger. I denne protokollen er det instrumentelle oppsettet av et integrert SRS og to-foton fluorescensmikroskop beskrevet for å visualisere cellulære strukturer i ryggmargen til levende mus.
