A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:11Introduction
- 04:44Odor dilution
- 08:20Preparation of larvae for tracking
- 10:38Preparation of plates for tracking
- 12:20Creation of odor gradient
- 17:07Tracking system
- 17:28Behavioral assay
- 20:24Single source assay
- 22:31Test of smell in individual larvae
- 23:33Multiple source assay
- 27:34Future directions
Neste artigo de vídeo, descrevemos um método novo que permite a construção de gradientes de odores com geometrias estável e controlável. Brevemente ilustrar como esses gradientes podem ser usados para triagem de defeitos olfativa (anosmia total e parcial) e estudar características mais sutis de comportamento de quimiotaxia.
Tags
High-resolution MeasurementOdor-driven BehaviorDrosophila LarvaeOlfactory ResponsesPetri DishesPeripheral Odor SourceDiffusion Of Odorant MoleculesStable GradientQuantitative CorrelationSensory InputsBehavioral ResponsesOdorant Stimulus ConditionsVideo ArticleConstruction Of Odorant GradientsStable And Controllable GeometriesScreen For Olfactory DefectsAnosmiaChemotaxis Behavior
