A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage

Min-Yu Chang; Hsi-Hao Wang; Li-Hung Chen; Jhen Gao; Shih-Yuan Hung; Yuan-Yow Chiou; Yi-Che Lee

doi:10.3791/63903

A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage

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JoVE Journal Medicine

A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage

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04:25 min

April 28, 2022

DOI: 10.3791/63903-v

Min-Yu Chang*^1,2, Hsi-Hao Wang*^1,2, Li-Hung Chen*¹, Jhen Gao*¹, Shih-Yuan Hung^1,2, Yuan-Yow Chiou^3,4, Yi-Che Lee^1,2,5

¹School of Medicine, College of Medicine,I-Shou University, ²Division of Nephrology, Department of Internal Medicine,E-DA Hospital, ³Department of Pediatrics,National Cheng Kung University Hospital, ⁴Institute of Clinical Medicine, College of Medicine,National Cheng Kung University, ⁵Division of Nephrology, Department of Internal Medicine,E-DA Dachang Hospital

The present protocol establishes a peritoneal dialysis (PD) mouse model of chlorhexidine gluconate (CG)-induced peritoneal fibrosis. The current model is simple and easy to use compared to other PD animal models.

Establishing animal model for peritoneal dialysis, PD, is very important. We hope it can improve the technology and the efficacy of PD therapy and eventually improve the patient's survival. This peritoneal dialysis mouse model is simple, easy to use, and visible compared to other peritoneal dialysis animal models.

A suitable peritoneal dialysis animal model can help researchers to study the peritoneal, physiological, and the pathological change during PD therapy. Demonstration of the procedure will be Fang-Ling Liao, a research assistant from my laboratory. To begin, prepare the chemical irritant by diluting 0.1%chlorhexidine gluconate in 15%ethanol.

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