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Rekonstituering av membranbundet minimal aktin cortices på støttede lipid dobbeltlag
JoVE Journal
Biology
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JoVE Journal Biology
Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

Rekonstituering av membranbundet minimal aktin cortices på støttede lipid dobbeltlag

DOI:
10.3791/63968-v

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11:55 min

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July 12, 2022

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, , ,
1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School,University of Warwick, 2National Centre for Biological Sciences,Tata Institute of Fundamental Research

Chapters

  • 00:05Introduction
  • 00:45Preparation of Sample Chambers
  • 02:59Preparation of Supported Lipid Bilayers
  • 04:31Addition of Membrane-Actin Linker
  • 05:27Quality Assessment of the Lipid Bilayer
  • 07:06Polymerization and Addition of Fluorescent Actin Filaments
  • 09:02Addition of Myosin II
  • 10:21Results: Understanding the Membrane-Cortex Interface of Cells
  • 11:12Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver dannelsen av støttede lipid-dobbeltlag og tilsetning av cytoskeletale filamenter og motorproteiner for å studere dynamikken i rekonstituerte, membranbundne cytoskeletale nettverk ved bruk av fluorescensmikroskopi.

Tags

Keywords: Membrane-tethered Actin CorticesSupported Lipid BilayersCytoskeletal ProteinsReconstitutionLight MicroscopyProtein AssemblyLipid Bilayer FormationUV Curable AdhesiveChamber Fabrication

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