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Bioengineering
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通过
侧向柔光照明促进脑类器官培养
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Facilitating Cerebral Organoid Culture
via
Lateral Soft Light Illumination
Please note that all translations are automatically generated.
Click here for the English version.
通过
侧向柔光照明促进脑类器官培养
DOI:
10.3791/63989-v
•
09:10 min
•
June 06, 2022
•
Bangzhu Chen
2
,
Qinrong Lin
2
,
Nengqing Liu
2
,
Diyu Chen
2
,
Yinghui Zhang
,
Xiaofang Sun
2
1
Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases
,
The Third Affiliated Hospital of Guangzhou Medical University
,
2
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes
,
3
School of Biotechnology and Health Sciences
,
Wuyi University
Chapters
00:04
Introduction
00:51
Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
02:54
Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
04:24
Neural Induction (Days 5 7)
05:25
Embedding in the Basement Membrane Matrix (Days 7 10)
06:52
Organoid Maturation (Days 10 40)
07:21
Results: Analysis of Cerebral Organoids
08:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
脑类器官为研究器官发育和人类疾病病理学提供了前所未有的机会。虽然脑类器官培养系统取得了巨大成功,但在应用这项技术方面仍然存在操作困难。本方案描述了一种促进介质改变和类器官转移的脑类器官程序。
Tags
Cerebral Organoids
Lateral Soft Light Illumination
IPSCs
EB Formation
Anti-adherence Rinsing Solution
Rho Kinase Inhibitor Y27632
Centrifugation
LED White Lights
Low Adhesion Plate
Medium Change
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