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Neuroscience

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JoVE Journal Neuroscience
Undersøgelse af drivere til antireward i afhængighedsadfærd med anatomisk specifikke enkeltcellegenekspressionsmetoder
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Undersøgelse af drivere til antireward i afhængighedsadfærd med anatomisk specifikke enkeltcellegenekspressionsmetoder

Article DOI: 10.3791/64014-v • 09:29 min • August 4th, 2022
August 4th, 2022 •
*1,2,3, *1, 1, 1
1Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, 2Sidney Kimmel Medical College, Thomas Jefferson University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine
* These authors contributed equally

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Kombinationen af laserfangstmikrodissektion og mikrofluidisk RT-qPCR giver anatomisk og bioteknisk specificitet ved måling af transkriptomet i enkeltneuroner og glia. Anvendelse af kreative metoder med et systems biologiske tilgang til psykiatrisk sygdom kan føre til gennembrud i forståelse og behandling såsom neuroinflammation antireward hypotesen i afhængighed.

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Neurovidenskab udgave 186 enkeltcelle genekspression laserfangstmikrodissektion mikrofluidisk qPCR afhængighed visceral-følelsesmæssig neuraxis neuroinflammation antireward
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