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体内小鼠海马体中小胶质细胞的慢性双光子成像

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

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体内小鼠海马体中小胶质细胞的慢性双光子成像

DOI:

10.3791/64104-v

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07:03 min

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July 06, 2022

•

Ryosuke Kamei, Shinji Urata, Hisato Maruoka, Shigeo Okabe

¹Department of Cellular Neurobiology, Graduate School of Medicine,The University of Tokyo

Chapters

00:04Introduction
00:36Implantation of the Observation Window
02:34Two‐Photon Microscopy Immediately After Surgery for Quality Check
03:55In Vivo Chronic Two‐Photon Imaging of Microglia Using CX3CR1‐GFP Mice
04:29Results: In Vivo Imaging of Microglia in CX3CR1‐GFP Mice
06:20Conclusion

Summary

Automatic Translation

本文描述了一种使用精确控制手术和双光子显微镜对小鼠海马CA1中静息小胶质细胞 进行慢性体内 观察的方法。

Tags

Keywords: Microglia Hippocampus In Vivo Two-photon Imaging Neurons Cranial Window External Capsule Alveus CA1 Two-photon Microscope

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