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Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor
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Neuroscience
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JoVE Journal Neuroscience
Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

DOI:
10.3791/65085-v

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07:21 min

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March 17, 2023

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, , , , , , , , ,
1Fraunhofer Project Center for Stem Cell Process Engineering,Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology,Saarland University, 6Facultad de Ciencias del Mar,Universidad Católica del Norte

Chapters

  • 00:04Introduction
  • 00:51Precultivation of hiPSCs in the Benchtop Bioreactor System (Day ‐2)
  • 02:53Differentiation of hiPSCs into Early Neurons (Day 0)
  • 03:29Cryopreservation of Early Neurons (Day 2)
  • 05:01Results: Assessing the Generation of hiPSC‐Derived Neurons Using the Benchtop Bioreactor
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.

Tags

Keywords: 3D Suspension BioreactorHuman Neurogenin 2-inducible NeuronsInduced Pluripotent Stem CellsIPSC CultureNeuronal DifferentiationHigh-throughput ScreeningRapid And Cost-efficient GenerationCardiomyocytesPre-cultivationTrypsinizationCell SingularizationCell CountingCell SuspensionCentrifugationFeeder-free IPSC Maintenance MediumROCK InhibitorBioreactor Cultivation

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