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Single Cell Elektroporatie in vivo binnen de Intact ontwikkeling van de hersenen
JoVE Journal
Biology
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JoVE Journal Biology
Single Cell Electroporation in vivo within the Intact Developing Brain
DOI:

13:31 min

July 11, 2008

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Chapters

  • 00:00Title
  • 00:52Introduction
  • 02:13Fabrication and Loading of Micropipettes
  • 04:02Single-Cell Electroporation Technique
  • 09:16Screening for Successfully Electroporated Cells
  • 10:16In Vivo 2-Photon Imaging of Neurons Fluorescently Labeled by Single-Cell Electroporation
  • 12:46Conclusion

Summary

Automatic Translation

Single-cell elektroporatie (SCE) is een gespecialiseerde techniek waardoor de levering van DNA of andere macromoleculen in individuele cellen in intact weefsel, ook in vivo preparaten. Hier hebben we detail de procedure voor SCE van een fluorescente kleurstof of plasmide DNA in neuronen in de intacte hersenen van de Xenopus laevis kikkervisje.

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