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Chapters
- 00:00Title
- 00:52Introduction
- 02:13Fabrication and Loading of Micropipettes
- 04:02Single-Cell Electroporation Technique
- 09:16Screening for Successfully Electroporated Cells
- 10:16In Vivo 2-Photon Imaging of Neurons Fluorescently Labeled by Single-Cell Electroporation
- 12:46Conclusion
Encelliga elektroporation (SCE) är en specialiserad teknik som möjliggör leverans av DNA eller andra makromolekyler i enskilda celler i intakta vävnader, inklusive in vivo förberedelser. Här har vi detalj förfarandet för SCE av en fluorescerande färg eller plasmid-DNA till nervceller i den intakta hjärnan i Xenopus laevis grodyngel.
Tags
Single-cell ElectroporationIn VivoIntact Developing BrainDNA DeliveryMacromoleculesIndividual CellsExperimental ManipulationsCell-autonomous EffectsGlobal TreatmentsIn Vivo Imaging TechniquesFluorescent MarkersCellular MorphologyCell GrowthIntracellular EventsXenopus Laevis TadpolesVideo ArticleProcedure For SCEFluorescent DyePlasmid DNANeuronsAlbino Xenopus TadpoleYield OptimizationTwo-photon Fluorescence Imaging
