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在人类胚胎干细胞的线粒体复杂的活动初探
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Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
在人类胚胎干细胞的线粒体复杂的活动初探
DOI:
10.3791/724-v
•
12:42 min
•
June 17, 2008
•
Ivan Khvorostov
,
Jin Zhang
,
Michael Teitell
1
David Geffen School of Medicine
,
University of California, Los Angeles
Chapters
00:03
hESCs and Mitochondrial Complex Activity
00:44
Introduction
01:41
Mitochondrial Isolation
04:08
Solubilizing the Crude Mitochondrial Pellet
05:42
Gel and Sample Preparation and Electrophoresis Conditions
09:47
In-Gel Activity Assays
12:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这段视频中,我们将向您可以分析人类胚胎干细胞的线粒体呼吸链复合物如何使用凝胶活性测定。
Tags
Mitochondrial Complex Activity
Human Embryonic Stem Cells
Cellular Metabolism
Homeostasis
Cell Signaling Network
Programmed Cell Death
ATP Production
Cytoplasmic Redox State
Ca2+ Balance
Fatty Acid Catabolism
Heme Synthesis
Nucleotide Synthesis
Steroid Hormone Synthesis
Amino Acid Synthesis
Iron-sulfur Cluster Assembly
Heat Production
Mitochondrial Genome Mutations
Aging Correlation
Cancer Development Correlation
Stem Cell Viability
Stem Cell Proliferation
Stem Cell Differentiation Capacity
Energy Status Of Stem Cells
Mitochondrial Properties Of Human Embryonic Stem Cells (hESCs)
Precursor Progeny Differentiation
Measurement Of Mitochondrial Respiratory Complexes
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