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Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
DOI:

14:42 min

June 24, 2008

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Chapters

  • 00:05Title
  • 01:45Embryo removal and placental dissection
  • 06:01Single-cell suspension of placenta
  • 10:22Embedding the fixed tissue
  • 12:25Expected tissue section results
  • 13:23Conclusion

Summary

Automatic Translation

We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta.

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