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Chapters
- 00:00Title
- 00:34Introduction
- 01:12Lysing Eggs and Collecting Crude Extract
- 03:36Fractionating Crude Egg Extracts and Collecting Cytoplasmic Extract
- 05:23Collecting the Light Membrane Fraction of the Egg Extract
- 07:02Conclusion
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
