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Journal
/
Immunology and Infection
/
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
DOI:
10.3791/1442-v
•
08:45 min
•
September 09, 2009
•
Suma Chakravarthy
,
André C. Velásquez
2
,
Gregory B. Martin
2
1
Boyce Thompson Institute for Plant Research
,
2
Plant Pathology and Plant-Microbe Biology
,
Cornell University
Chapters
00:05
Title
00:08
Introduction
00:34
Preparing for the PTI Assay
03:26
Induction of PTI
05:20
Challenge Inoculation
06:32
Scoring for the Breakdown of PTI
07:33
Representative Results
08:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
A cell death-based assay for PTI in Nicotiana benthamiana plants is described.
Tags
Assay
Pathogen-Associated Molecular Pattern
PAMP
Triggered Immunity
PTI
Plants
Pattern Recognition Receptors
PRRs
Plasma Membranes
Microbial Features
Pathogen-associated Molecular Patterns
FLS2-dependent Pathway
Flg22
Bacterial Flagellin
Virulence Factors
Effectors
Cell Death-based Assay
N. Benthamiana
Model System
Plant-pathogen Interactions
Article
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