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RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
JoVE Journal
Biology
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JoVE Journal Biology
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
DOI:

15:21 min

August 07, 2009

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Chapters

  • 00:05Title
  • 00:41Introduction
  • 01:03Extraction of Total RNA using TRIzol Reagent
  • 03:55RNA Cleanup using the Qiagen RNEasy Mini Kit
  • 07:57cDNA Synthesis using the Invitrogen SuperScript First-Strand System
  • 10:28cDNA Isolation and Precipitation
  • 13:50Representative Results/Outcome
  • 14:57Conclusion

Summary

Automatic Translation

The isolation of high quality, intact RNA is an essential step in many laboratory protocols. Here, we demonstrate RNA extraction from whole zebrafish embryos and cDNA synthesis for subsequent application in various experimental procedures including gene expression microarray analysis.

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