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Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
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Chapters
- 00:05Title
- 00:58Introduction
- 01:19Introduction to DMD Chips and General Design Considerations
- 04:12Illumination of the DMD
- 06:14Operation of the System
- 07:16Representative Results Demonstrating Patterned Photostimulation with DMDs to Investigate Dendritic Integration across Branch Points
- 08:43Conclusion
Digital micromirror devices (DMD) can generate complex patterns in time and space with which to control neuronal excitability. Issues relevant to the design, construction, and operation of DMD systems are discussed. Such a system enabled the demonstration of non-linear integration across distal dendritic branch points.
Tags
Patterned PhotostimulationDigital Micromirror DevicesDendritic IntegrationNeuronal ExcitabilityLight-sensitive EffectorsChannel-rhodopsinCaged NeurotransmittersExperimental NeuroscienceConfocal Microscopy2-photon MicroscopyDiffraction Limited SpotScanningPhotostimulationImagingSpatial PatternTarget ProjectionHardware ComponentsUpright MicroscopesUV PhotolysisVisible Light SystemOptogenetic Experiments
