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JoVE Journal
Immunology and Infection

This content is Open Access.

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
 

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

Article DOI: 10.3791/2770-v 07:30 min December 1st, 2011
December 1st, 2011

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Summary

The protocol describes a high-throughput approach to determining structures of membrane proteins using cryo-electron tomography and 3D image processing. It covers the details of specimen preparation, data collection, data processing and interpretation, and concludes with the production of a representative target for the approach, the HIV-1 Envelope glycoprotein. These computational procedures are designed in a way that enables researchers and students to work remotely and contribute to data processing and structural analysis.

Tags

HIV Molecular Structures Cryo-Electron Tomography Automated Sub-tomogram Averaging HIV Envelope Glycoproteins CD4+ T-cells Immune System Acquired Immunodeficiency Syndrome (AIDS) Spike Protein CD4 Receptor Co-receptor Protein Interactions HIV Infection Pathway HIV Research HIV Vaccine Cryo-electron Tomography 3D Image Processing Viral Membrane Proteins
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