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Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination
DOI:

07:42 min

August 01, 2011

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Chapters

  • 00:05Title
  • 00:58Preparing for Injection
  • 02:46Injection Procedure and Pup Recovery
  • 05:07Equipment Cleaning
  • 05:47Post-injection Marker Expression
  • 07:28Conclusion

Summary

Automatic Translation

An in vivo method to test gene function in postnatal brain is described. Recombinant AAVs expressing Cre and/or a fluorescent protein are injected into neonatal mouse brain. Mosaic gene inactivation and sparse neuronal labeling are achieved, allowing rapid analysis of gene function in processes critical to neural circuit development.

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