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Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination
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Chapters
- 00:05Title
- 00:58Preparing for Injection
- 02:46Injection Procedure and Pup Recovery
- 05:07Equipment Cleaning
- 05:47Post-injection Marker Expression
- 07:28Conclusion
An in vivo method to test gene function in postnatal brain is described. Recombinant AAVs expressing Cre and/or a fluorescent protein are injected into neonatal mouse brain. Mosaic gene inactivation and sparse neuronal labeling are achieved, allowing rapid analysis of gene function in processes critical to neural circuit development.
Tags
Mosaic AnalysisGene FunctionPostnatal Mouse Brain DevelopmentVirus-based Cre RecombinationNormal Brain FunctionEmbryonic DevelopmentNeuronal PathwaysPostnatal DevelopmentNeural CircuitsNeurological DisordersPsychiatric DisordersAutismSchizophreniaPrenatal BrainDevelopmental ProcessesGene DeletionMouse ModelsFloxed AllelesTransgenic AllelesConditional DeletionGene Function AnalysisVirally-expressed CreRecombinant Adeno-associated Viruses (rAAVs)
