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Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
DOI:

12:00 min

December 28, 2011

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Chapters

  • 00:05Title
  • 01:35Preparing the Microinjector
  • 02:42Preparing the Animal and Performing the Stereotaxic Injection
  • 04:53Preparing the Miniature LED for Implantation
  • 06:54Chronic LED Implantation for in vivo Optogenetic Stimulation
  • 09:29Injected Cells and Implantation
  • 10:46Conclusion

Summary

Automatic Translation

Adult-born neurons of the olfactory bulb can be optogenetically controlled using Channelrhodopsin2-expressing lentiviral injection in the rostral migratory stream and chronic photostimulation with an implanted miniature LED.

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