Journal
/
/
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
DOI:

07:52 min

July 23, 2012

,

Chapters

  • 00:05Title
  • 01:24Sensitizing and Intranasal Challenge of Mice with Ovalbumin
  • 02:39Preparation of Single-cell Suspension from the Lungs and the Draining Lymph Nodes
  • 05:37Results: OVA Sensitization and Challenge Induces an Increase in CD11c+ Cell Trafficking to Murine Mediastinal Lymph Nodes
  • 07:33Conclusion

Summary

Automatic Translation

We describe a strategy to monitor maturation and migration of pulmonary dendritic cells in response to ovalbumin in the setting of ovalbumin induced allergic airway inflammation. This strategy can be modified to assess migration of pulmonary dendritic cells in settings of infection.

Related Videos

Read Article