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Chapters
- 00:05Title
- 01:46Dissociation of Zebrafish Embryos and Plating of Myofibers
- 03:36Mounting Coverslips
- 04:22Representative Myofiber Imaging Results
- 05:08Conclusion
Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.
