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Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion
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Bioengineering
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JoVE Journal Bioengineering
Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

DOI:
10.3791/50604-v

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10:30 min

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September 04, 2013

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, ,
1Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology,Ohio University, 2Biomedical Engineering Program,Ohio University

Chapters

  • 00:05Title
  • 01:17Installation of the Dual Camera System
  • 02:47Camera Alignment
  • 04:34Preparation of Cells, Reactive Substrate, and Parallel Plate Flow Chamber
  • 05:50Two-color Image Acquisition
  • 08:27Representative Imaging Results
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.

Tags

Dual Camera Emission Splitting SystemMulti-color Immunofluorescence MicroscopyParallel Plate Flow ChamberReal-time Live Cell AnalysisFluorescently Labeled CellsCell AdhesionCancer Metastasis

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