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The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
JoVE Journal
Biology
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JoVE Journal Biology
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
DOI:

10:57 min

March 11, 2014

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Chapters

  • 00:05Title
  • 01:32Agar Bridge and Cut-open Rig Preparation
  • 03:54Cut-open Procedure
  • 09:48Results: The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique with Fluorometry
  • 10:27Conclusion

Summary

Automatic Translation

The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.

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