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Journal
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Biology
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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
JoVE Journal
Biology
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JoVE Journal
Biology
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
DOI:
10.3791/51408-v
•
09:03 min
•
May 29, 2014
•
HoTae Lim
,
In Young Choi
,
Gabsang Lee
1
Institute for Cell Engineering, Department of Neurology and Neuroscience
,
Johns Hopkins University School of Medicine
Chapters
00:05
Title
01:20
Preparing hESCs for FACS Purification
02:26
Lysis of FACS-purified Single Cell in Each Well of a 96-well Plate
03:28
Reverse Transcription
04:29
Amplification
05:26
qRT-PCR Performance
06:21
Results: qRT-PCR is an Efficient Method for Profiling Single Cell Gene Expression
08:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Single cell gene expression assay is needed for understanding stem cell heterogeneities.
Tags
Single Cell
Transcriptome
QRT-PCR
Heterogeneity
Stem Cell
Differentiation
OCT4
NANOG
Gene Expression
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