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JoVE Journal
Biology

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锌指核酸酶增强基因打靶的人胚胎干细胞
 
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锌指核酸酶增强基因打靶的人胚胎干细胞

Article DOI: 10.3791/51764-v 12:13 min August 23rd, 2014
August 23rd, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

记者的细胞系提供了可视化,跟踪和隔离,从不同的人群感兴趣的细胞的方法。然而,基因靶向用常规的同源重组在人胚胎干细胞是非常低效的。在这里,我们将介绍使用锌指核酸酶增强同源重组靶向中枢神经系统中脑特异性转录因子PITX3基因与绿色荧光蛋白。

Tags

分子生物学,第90,电穿孔,人类胚胎干细胞,基因组编辑,报道细胞系,中脑多巴胺能神经元
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