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Biology

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A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors
 

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

Article DOI: 10.3791/51984-v 12:40 min December 7th, 2014
December 7th, 2014

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Summary

Emergence of genetic resistance against kinase inhibitor therapy poses significant challenge for effective cancer therapy. Identification and characterization of resistant mutations against a newly developed drug helps in better clinical management and next generation drug design. Here, we describe our protocol for in vitro screening and validation of resistant mutations.

Tags

Screening Validation Resistant Mutations Kinase Inhibitors BCR/ABL Chronic Myeloid Leukemia Imatinib Oncogenic Kinases Drug Resistance Mutations Personalized Medicine Retroviral Vector Next Generation Inhibitors Ruxolitinib JAK2 In Vitro Screening Methods
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