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Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
DOI:

08:02 min

September 21, 2014

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Chapters

  • 00:05Title
  • 01:09General Preparations
  • 02:41Dissection and Preparation of Tissue Slices
  • 04:57Tissue Microdissection and Cell Dissociation, Sorting, and Plating
  • 06:46Results of Isolating and Culturing NEPs
  • 07:43Conclusion

Summary

Automatic Translation

Nestin-expressing progenitors are a newly identified population of neuronal progenitors in the developing cerebellum. Using the microdissection technique presented here in combination with fluorescent-activated cell sorting, this cell population can be purified with no contamination from other cerebellar regions and can be cultured for further studies.

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