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Long-term Time Lapse Imaging of Mouse Cochlear Explants
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Neuroscience
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JoVE Journal Neuroscience
Long-term Time Lapse Imaging of Mouse Cochlear Explants

Long-term Time Lapse Imaging of Mouse Cochlear Explants

DOI:
10.3791/52101-v

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10:43 min

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November 02, 2014

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,
1Biological Sciences Platform,Sunnybrook Research Institute, 2Department of Otolaryngology – Head and Neck Surgery,University of Toronto, 3Department of Laboratory Medicine and Pathobiology,University of Toronto

Chapters

  • 00:05Title
  • 01:31Culturing Embryonic Cochleae and Explants
  • 05:56Live Imaging
  • 08:57Results: Cochlear Explant Time Lapse
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

Live imaging of the embryonic mammalian cochlea is challenging because the developmental processes at hand operate on a temporal gradient over ten days. Here we present a method for culturing and then imaging embryonic cochlear explant tissue taken from a fluorescent reporter mouse over five days.

Tags

Cochlear ExplantTime-lapse ImagingLive ImagingEmbryonic MouseMammalian CochleaGene ExpressionFluorescent MicroscopyBright Field MicroscopyIncubator MicroscopeSox2Prosensory Cells

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