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Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture
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Bioengineering
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JoVE Journal Bioengineering
Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture

DOI:
10.3791/53475-v

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10:55 min

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January 11, 2016

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, ,
1Department of Bio and Brain Engineering,Korea Advanced Institute of Science and Technology (KAIST)

Chapters

  • 00:05Title
  • 01:18Preparation of the Micropatterned Molds
  • 03:48Fabrication of Freestanding Cellular Hydrogel Sheets
  • 07:03Assembly of Multi-layered Hydrogel Sheets
  • 09:29Results
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the fabrication of micropatterned hydrogel sheets using a simple process, which can be assembled and manipulated in a freestanding form. Using these modular hydrogel sheets, a simple macro-scaled 3D cell culture system can be generated with a controlled cellular microenvironment.

Tags

3D Cell CultureModular Hydrogel SheetsMicropatterned 3D Cellular ArchitectureIn Vitro Cell-based AssaysPDMSPhotolithographyHydrophilic SurfaceSurfactant Solution

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