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In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
JoVE Journal
Chemistry
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JoVE Journal Chemistry
In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
DOI:

09:48 min

February 15, 2016

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Chapters

  • 00:05Title
  • 01:03Cleaning and Sterilization of the SALVI Microchannel
  • 02:05Immobilization of the Protein Film in SALVI
  • 02:50Install SALVI into the ToF-SIMS Loadlock Chamber
  • 04:05ToF-SIMS Data Acquisition
  • 07:35Results: Dynamic Liquid Surface and Liquid-solid Interface Analysis
  • 09:02Conclusion

Summary

Automatic Translation

This work presents a protocol for liquid handling and sample introduction to a microchannel for in situ time-of-flight secondary ion mass spectrometry analysis of protein biomolecules in an aqueous solution.

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